Análise da atividade antimicrobiana de quatro pastas endodônticas sobre microorganismos removidos da cavidade pulpar de molares decíduos necrosados

Abstract

The aim of this study was to analyze in vitro the antimicrobial activity of four

materials (CTZ, Guedes-Pinto, Calen PMCC®

of deciduous teeth on some microorganisms removed from the pulp cavity of necrotic

primary molars. We selected 06 children aged 6 to 10 years old, each presented with

a deciduous molar was necrotic. In the clinical phase, the involved teeth were

accessed and an absorbent paper cone inserted inside the root canal, then the

contaminated cones were placed in a tube containing 3 ml of TSB. The tubes were

sent to the laboratory and all involved teeth treated and filled with paste CTZ.

Coronal restoration was performed with cement glass ionomer and the teeth were in

attendance. In the laboratory phase, the tubes were incubated in TSB bacteriological

incubator at 37 °C for a period of 24 hours. Then, they were subcultured in Petri

plaques containing culture media: blood agar, mannitol salt agar and chocolate agar

CLED. The media were incubated in bacteriological incubator at 37 °C for 24 hours,

except the chocolate agar that was placed in an anaerobic jar and incubated in the

same conditions. After the growth and identification of bacteria, with the aid of a

sterile swab, were sown in Petri plaques containing Mueller Hinton agar. The folders

(CTZ, Guedes-Pinto, OZE, Calen PMCC®

an oven at 37 °C for 24 hours to verify and measure the inhibition of bacterial growth.

The microorganisms identified were K. pneumonae, S. aureus, E. coli, E. faecalis

and bacteria grown in anaerobic conditions. In the agar diffusion test of the filling

materials, folders CTZ and Guedes-Pinto presented the best results in vitro, followed

by cement and zinc oxide eugenol paste and Calen PMCC®

, OZE) used in obturation of root canals

) were placed in medium and incubated in

. It was concluded that

root canal filling pastes in primary teeth with the antibiotic in its composition were

more effective in eliminating bacteria removed from endodontic infections.